After injecting 1.5 ng of MO, approximately 30.0% of the embryos exhibited reduced birefringence. Image representative of three independent experiments. Nuclear distribution and shape indicate that hydrostatic pressure of the notochord is decreased in spzl mutants. Annotation: yes Show organism ... All databases (78756) Download RDF. The Ensembl project produces genome databases for vertebrates and other eukaryotic species, and makes this information freely available online. GBrowse provides a graphical, interactive and customizable web interface to explore the zebrafish genome, including information about genome annotations and the positions of genes on the chromosomes. (F) AICAR treatment reduced the percentage of BR-reduced fish. These databases may hold many species genomes, or a single model organism genome. Sequence archive. Asterisks mark fusion events. Systems used to automatically annotate proteins with high accuracy: Select one of the options below to target your search: Select item(s) and click on "Add to basket" to create your own collection here (400 entries max). The zebrafish reference genome sequence and its relationship to the human genome. Of the untreated MO morphants, 31.1% were BR reduced. Browse a Genome. Can't find a genome assembly? ( B) Genome organization of the critical genomic interval near the spzl locus. (C) Panels represent the results of the birefringence assay (BR). β-Actin: loading control. d Western blot of the endogenous Chk1 and Wee1 proteins in WT and def−/− mutant embryos at 5dpf. Your input has been emailed to ZFIN curators who may contact you if Select your default SMART mode. The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the Try our FAQs or glossary. (E) Liquid chromatography-mass spectrometry (LC-MS) analysis of tRNA modification. Numerator/denominator: number of embryos displayed the shown phenotype over total number of genotyped embryos. This section now includes direct links to zebrafish gene pages in Entrez Gene and in the Sanger Institute's Vertebrate Genome Annotation database (Vega). GENOME: Danio rerio (zebrafish) Help: Entry: T01004 Complete Genome : Name: dre, DANRE, 7955. A nonsense mutation generates an early stop codon in dstyk. The Δ19Δ14 mutation creates a new early stop codon in the presumed variant initiated from M94 after C120, therefore, capn3bΔ19Δ14 is likely a null allele. Want to use GRCh38? ( F–H) Bright field images of spzl-/-and WT sibling, spzl;dstykpd1133-/- and WT sibling, dstykpd1133 homozygous mutant and WT sibling, scale bars = 1 mm. a Diagram showing the Capn3 recognition motif (left panel) and the locations of this motif in zebrafish, human and mouse Chk1 (middle panel) and Wee1 (right panel) proteins. Find Position - … The Zebrafish Information Network is an online biological database of information about the zebrafish (Danio rerio).The zebrafish is a widely used model organism for genetic, genomic, and developmental studies, and ZFIN provides an integrated interface for querying and displaying the large volume of data generated by this research. Scale bar = 20 µm ( G) Sphericity of WT and spzl-/- nuclei at 14 dpf. ... Zebrafish Zv9. gabra1 expression in the developing zebrafish. DAPI: stain nuclei. This BED file contains all CRISPR/Cas9 targets in the zebrafish genome (danRer11) that fit the criteria of at least three mismatches overall. (A) Schematic representation of the anti-codon loop of a tRNA, indicating position 34 (red arrow) where 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) is located. C120, Capn3b activity center. Statistical significance was set at *p < 0.05. 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